Hotspot

trnS-GCU-trnG-UCC

LSC · 7464-8418 · 955 bp

Back to Muiriantha

Pi 0.0332

MarkerSeek score 68.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 31
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.0	57.2	1031-1034 bp	1.0	61.2
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.0	57.1	1030-1033 bp	1.0	60.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.2	57.2	1089-1092 bp	1.0	59.8
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	GGGGATTACTTGAACGAATCAC	50.0	45.5	58.2	57.1	1088-1091 bp	1.0	59.5
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	AGGGGATTACTTGAACGAATCA	50.0	40.9	58.3	57.2	1028-1031 bp	1.0	59.2