primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGACGCTTTAATCCACTCA	AGGGGATTACTTGAACGAATCA	20	22	50.0	40.9	58.0	57.2	0.0	0.0	2.841676	7464	8418	1031-1034	1.000000	61.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCGACGCTTTAATCCACTCA	GGGGATTACTTGAACGAATCAC	20	22	50.0	45.5	58.0	57.1	0.0	0.0	2.881906	7464	8418	1030-1033	1.000000	60.9
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	AGGGGATTACTTGAACGAATCA	20	22	50.0	40.9	58.2	57.2	0.0	0.0	2.988441	7464	8418	1089-1092	1.000000	59.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	GGGGATTACTTGAACGAATCAC	20	22	50.0	45.5	58.2	57.1	0.0	0.0	3.028671	7464	8418	1088-1091	1.000000	59.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGCTTTAATCCACTCAGCC	AGGGGATTACTTGAACGAATCA	20	22	50.0	40.9	58.3	57.2	0.0	0.0	3.098026	7464	8418	1028-1031	1.000000	59.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAAATCAATTGCGTCCAA	20	20	50.0	40.0	58.0	56.2	0.0	17.3	1.805330	9223	9410	315-315	1.000000	69.9
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAAATCAATTGCGTCCAA	20	20	55.0	40.0	58.1	56.2	0.0	17.3	1.834166	9223	9410	318-318	1.000000	69.6
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	ATGGAAATCAATTGCGTCCA	20	20	50.0	40.0	58.0	56.0	0.0	20.2	2.037301	9223	9410	316-316	1.000000	68.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	ATGGAAATCAATTGCGTCCA	20	20	55.0	40.0	58.1	56.0	0.0	20.2	2.066138	9223	9410	319-319	1.000000	67.7
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	TGGAAATCAATTGCGTCCAA	20	20	55.0	40.0	57.7	56.2	0.0	17.3	2.109180	9223	9410	319-319	1.000000	67.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	CTGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.269981	9483	9675	347-347	1.000000	79.9
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CTGAAAGGCGTCCATTGTCT	GAAGCCCTTTTGCAAGAAGC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.398228	9483	9675	338-338	1.000000	78.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	CTGAAAGGCGTCCATTGTCT	AGCCCTTTTGCAAGAAGCTA	20	20	50.0	45.0	58.2	57.7	0.0	11.2	0.479315	9483	9675	336-336	1.000000	78.3
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	ACTGAAAGGCGTCCATTGTC	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.559737	9483	9675	348-348	1.000000	77.6
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GACTGAAAGGCGTCCATTGT	GAGGAAGCAGAAGCCCTTTT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.559737	9483	9675	349-349	1.000000	77.6
atpA-atpF_p1	atpA-atpF	1	CCGTCGCCTACTTGAAGTAC	TGACATATTTGGGGCGATGA	20	20	55.0	45.0	58.1	57.0	0.0	0.0	1.113679	11200	11258	209-209	1.000000	72.6
atpA-atpF_p2	atpA-atpF	2	GTCGCCTACTTGAAGTACGG	TGACATATTTGGGGCGATGA	20	20	55.0	45.0	58.1	57.0	13.2	0.0	1.113679	11200	11258	207-207	1.000000	72.6
atpA-atpF_p3	atpA-atpF	3	CCGTCGCCTACTTGAAGTAC	TGACATATTTGGGGCGATGAA	20	21	55.0	42.9	58.1	57.7	0.0	0.0	1.383957	11200	11258	209-209	1.000000	70.5
atpA-atpF_p4	atpA-atpF	4	GTCGCCTACTTGAAGTACGG	TGACATATTTGGGGCGATGAA	20	21	55.0	42.9	58.1	57.7	13.2	0.0	1.383957	11200	11258	207-207	1.000000	70.5
atpA-atpF_p5	atpA-atpF	5	GTCGCCTACTTGAAGTACGG	TTGACATATTTGGGGCGATGA	20	21	55.0	42.9	58.1	57.7	13.2	0.0	1.383957	11200	11258	208-208	1.000000	70.5
atpF-atpH_p1	atpF-atpH	1	GCCAGTGAACCAAAGAAACG	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.805521	12601	13117	663-673	1.000000	77.9
atpF-atpH_p2	atpF-atpH	2	GCCAGTGAACCAAAGAAACG	CGAGGCGGAGGGAAAAATAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.822944	12601	13117	668-678	1.000000	77.7
atpF-atpH_p3	atpF-atpH	3	GCCAGTGAACCAAAGAAACG	GAGGCGGAGGGAAAAATACG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.822944	12601	13117	667-677	1.000000	77.7
atpF-atpH_p4	atpF-atpH	4	GCCAGTGAACCAAAGAAACG	GCGGAGGGAAAAATACGAGG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.822944	12601	13117	664-674	1.000000	77.7
atpF-atpH_p5	atpF-atpH	5	GCCAGTGAACCAAAGAAACG	GGCGGAGGGAAAAATACGAG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.822944	12601	13117	665-675	1.000000	77.7
petN-psbM_p1	petN-psbM	1	ACATTTTCCCTTTCGCTCGT	TCGTTCTAGTTCCTACTGCCT	20	21	45.0	47.6	58.1	57.9	0.0	0.0	1.190913	28867	30185	1413-1429	1.000000	73.6
petN-psbM_p2	petN-psbM	2	CCCTTTCGCTCGTAGTATGG	TCGTTCTAGTTCCTACTGCCT	20	21	55.0	47.6	57.9	57.9	0.0	0.0	1.218787	28867	30185	1406-1422	1.000000	73.4
petN-psbM_p3	petN-psbM	3	CCTTTCGCTCGTAGTATGGG	TCGTTCTAGTTCCTACTGCCT	20	21	55.0	47.6	57.9	57.9	0.0	0.0	1.218787	28867	30185	1405-1421	1.000000	73.4
petN-psbM_p4	petN-psbM	4	CTTTCGCTCGTAGTATGGGG	TCGTTCTAGTTCCTACTGCCT	20	21	55.0	47.6	57.9	57.9	0.0	0.0	1.218787	28867	30185	1404-1420	1.000000	73.4
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	TCGTTCTAGTTCCTACTGCCT	20	21	50.0	47.6	58.2	57.9	0.0	0.0	1.234471	28867	30185	1451-1467	1.000000	73.1
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CGGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.4	58.1	3.7	0.0	0.421687	36923	37442	678-679	1.000000	78.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CGGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.4	58.1	3.7	0.0	0.421687	36923	37442	679-680	1.000000	78.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CGGATGGTTGGTCGAGTAAC	AGTCCTTTACTCTGGCGGAT	20	20	55.0	50.0	58.4	58.1	3.7	0.0	0.495649	36923	37442	704-705	1.000000	78.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TTTGCTTCTCCGGATGGTTG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.5	58.1	3.6	0.0	0.525050	36923	37442	688-689	1.000000	77.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTTGCTTCTCCGGATGGTTG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.5	58.1	3.6	0.0	0.525050	36923	37442	689-690	1.000000	77.8
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	GCTACCAAACTGCTCTACCC	GGGGGCAACAAGATCAAGTT	20	20	55.0	50.0	58.0	58.4	0.0	0.0	0.393000	37751	37913	212-214	1.000000	79.9
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	AAGGTTATGAGCCTTGCGAG	GGGGGCAACAAGATCAAGTT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.397250	37751	37913	231-233	1.000000	79.6
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	TTTGAACCCGTGACCTCAAG	GGGGGCAACAAGATCAAGTT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.405618	37751	37913	248-250	1.000000	79.3
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	AAAATGTTGCTGTTGCGGAG	GGGGGCAACAAGATCAAGTT	20	20	45.0	50.0	57.9	58.4	0.0	0.0	0.506008	37751	37913	274-276	1.000000	78.3
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	GATTTGAACCCGTGACCTCA	GGGGGCAACAAGATCAAGTT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.546323	37751	37913	250-252	1.000000	78.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CAACTAGAGAAAAGCCGGCT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.9	58.0	27.1	0.0	0.105228	47398	48301	1009-1021	1.000000	83.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47398	48301	954-966	1.000000	83.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CAACTAGAGAAAAGCCGGCT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.9	58.1	27.1	0.0	0.170037	47398	48301	1017-1029	1.000000	83.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47398	48301	962-974	1.000000	83.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ACAACTAGAGAAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	21.9	0.0	0.194940	47398	48301	1010-1022	1.000000	83.0
accD-psaI_p1	accD-psaI	1	TATTGCAGTTCCACGCCTTT	AAAGAAGCCATTGCCATTGC	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.199250	59144	59853	840-861	1.000000	82.4
accD-psaI_p2	accD-psaI	2	TTATTGCAGTTCCACGCCTT	AAAGAAGCCATTGCCATTGC	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.199250	59144	59853	841-862	1.000000	82.4
accD-psaI_p3	accD-psaI	3	TATTGCAGTTCCACGCCTTT	TTGCCATTGCCGGAAATACT	20	20	45.0	45.0	58.1	58.2	0.0	7.5	0.249707	59144	59853	830-851	1.000000	82.0
accD-psaI_p4	accD-psaI	4	TTATTGCAGTTCCACGCCTT	TTGCCATTGCCGGAAATACT	20	20	45.0	45.0	58.1	58.2	0.0	7.5	0.249707	59144	59853	831-852	1.000000	82.0
accD-psaI_p5	accD-psaI	5	TATTGCAGTTCCACGCCTTT	ATGGAGGGAAAGCTGTTGAG	20	20	45.0	50.0	58.1	57.5	0.0	0.0	0.599608	59144	59853	789-810	1.000000	79.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAAGCGGCTAGGAGAAAACC	20	20	50.0	50.0	57.9	58.2	6.4	1.4	0.228828	65190	66577	1530-1536	1.000000	80.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AAAGCGGCTAGGAGAAAACC	20	20	50.0	50.0	57.8	58.2	24.9	1.4	0.333837	65190	66577	1453-1459	1.000000	79.5
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAAGCGGCTAGGAGAAAACC	20	20	45.0	50.0	58.2	58.2	0.0	1.4	0.348515	65190	66577	1539-1545	1.000000	79.3
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AAAGCGGCTAGGAGAAAACC	20	20	50.0	50.0	58.2	58.2	0.0	1.4	0.361420	65190	66577	1544-1550	1.000000	79.2
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AAAGCGGCTAGGAGAAAACC	20	20	50.0	50.0	57.8	58.2	0.0	1.4	0.364845	65190	66577	1538-1544	1.000000	79.2
rps11-rpl36_p1	rps11-rpl36	1	AACCAATCCGTCCAGTCCTA	AAAATGTCGACTGATCCGCA	20	20	50.0	45.0	58.1	57.9	0.0	17.9	0.146322	80222	80351	259-261	1.000000	81.3
rps11-rpl36_p2	rps11-rpl36	2	CGACGTCTACTTTTACGCGA	AAAATGTCGACTGATCCGCA	20	20	50.0	45.0	58.1	57.9	15.9	17.9	0.223803	80222	80351	278-280	1.000000	80.5
rps11-rpl36_p3	rps11-rpl36	3	TCGACGTCTACTTTTACGCG	AAAATGTCGACTGATCCGCA	20	20	50.0	45.0	58.1	57.9	14.7	17.9	0.223803	80222	80351	279-281	1.000000	80.5
rps11-rpl36_p4	rps11-rpl36	4	CTTTTACGCGAACCAATCCG	AAAATGTCGACTGATCCGCA	20	20	50.0	45.0	57.8	57.9	15.9	17.9	0.246986	80222	80351	269-271	1.000000	80.4
rps11-rpl36_p5	rps11-rpl36	5	AACCAATCCGTCCAGTCCTA	GCAGGAGGGGACGGATTATA	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.412099	80222	80351	242-244	1.000000	79.3
rpl14-rpl16_p1	rpl14-rpl16	1	CCGCTATTGTCCGCTACATT	CATCGCTCGAAGGGCTATTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.263055	81703	81824	239-249	1.000000	79.5
rpl14-rpl16_p2	rpl14-rpl16	2	CCGCTATTGTCCGCTACATT	ATCGCTCGAAGGGCTATTTC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.290478	81703	81824	238-248	1.000000	79.3
rpl14-rpl16_p3	rpl14-rpl16	3	CCGCTATTGTCCGCTACATT	CAATAGCGGCGTCCAAAATG	20	20	50.0	50.0	58.1	58.2	0.0	9.4	0.307808	81703	81824	219-229	1.000000	79.3
rpl14-rpl16_p4	rpl14-rpl16	4	CCGCTATTGTCCGCTACATT	TCAATAGCGGCGTCCAAAAT	20	20	50.0	45.0	58.1	58.3	0.0	9.4	0.388903	81703	81824	220-230	1.000000	78.7
rpl14-rpl16_p5	rpl14-rpl16	5	CCGCTATTGTCCGCTACATT	ATTTCAATAGCGGCGTCCAA	20	20	50.0	45.0	58.1	58.3	0.0	9.4	0.388903	81703	81824	223-233	1.000000	78.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.172709	114158	114788	728-753	1.000000	83.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.174080	114158	114788	729-754	1.000000	83.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGTTGAAGGCTTTTTCGTT	TGCCTCGAGTAAATCCAACG	20	20	45.0	50.0	58.2	58.0	0.0	12.3	0.169462	114158	114788	1071-1097	1.000000	82.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGTTGAAGGCTTTTTCGTT	CTTGGTGCCGTTATAGTGCT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.189055	114158	114788	1191-1217	1.000000	81.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGTTGAAGGCTTTTTCGTT	CCTCGAGTAAATCCAACGGG	20	20	45.0	55.0	58.2	58.1	0.0	12.3	0.228947	114158	114788	1069-1095	1.000000	81.8
ccsA-ndhD_p1	ccsA-ndhD	1	AGGATTACATAGTTATGGTTCA	AGTGGACAAGACCGAAGCTA	22	20	31.8	50.0	52.0	58.4	4.3	0.0	8.358637	115922	116224	374-374	1.000000	44.9
ccsA-ndhD_p2	ccsA-ndhD	2	GGAATAGGATTACATAGTTATGGTTCA	AGTGGACAAGACCGAAGCTA	27	20	33.3	50.0	56.8	58.4	4.3	0.0	8.603807	115922	116224	379-379	1.000000	44.9
ccsA-ndhD_p3	ccsA-ndhD	3	AGGATTACATAGTTATGGTTCA	GTGGACAAGACCGAAGCTAT	22	20	31.8	50.0	52.0	57.3	4.3	0.0	8.662475	115922	116224	373-373	1.000000	44.9
ccsA-ndhD_p4	ccsA-ndhD	4	AGGATTACATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	22	20	31.8	50.0	52.0	57.4	4.3	0.0	8.637011	115922	116224	398-398	1.000000	44.6
ccsA-ndhD_p5	ccsA-ndhD	5	AGGATTACATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	22	20	31.8	50.0	52.0	57.3	4.3	0.0	8.644240	115922	116224	397-397	1.000000	44.6
ndhD-psaC_p1	ndhD-psaC	1	CGCGGCTATCGGAAAAACTA	ACTAGAAGCATGGGTCTAGCT	20	21	50.0	47.6	58.4	58.0	0.0	0.0	1.476141	117698	117892	240-246	1.000000	73.1
ndhD-psaC_p2	ndhD-psaC	2	CGCGGCTATCGGAAAAACTA	ACTAGAAGCATGGGTCTAGC	20	20	50.0	50.0	58.4	56.4	0.0	0.0	2.018607	117698	117892	240-246	1.000000	68.8
ndhD-psaC_p3	ndhD-psaC	3	GCGGCTATCGGAAAAACTAC	ACTAGAAGCATGGGTCTAGCT	20	21	50.0	47.6	57.0	58.0	0.0	0.0	2.051830	117698	117892	239-245	1.000000	68.5
ndhD-psaC_p4	ndhD-psaC	4	CGCGGCTATCGGAAAAACTA	AACTAGAAGCATGGGTCTAGC	20	21	50.0	47.6	58.4	57.2	0.0	0.0	2.265763	117698	117892	241-247	1.000000	66.8
ndhD-psaC_p5	ndhD-psaC	5	CGCGGCTATCGGAAAAACTA	ACAACTAGAAGCATGGGTCT	20	20	50.0	45.0	58.4	56.1	0.0	0.0	2.290754	117698	117892	243-249	1.000000	66.6
ycf1_p1	ycf1	1	TCTCTACGGCGTTTAGGGAA	CCCCGTCGTTTGTCTGTAAA	20	20	50.0	50.0	58.2	58.1	21.2	0.0	0.293419	124889	125008	239-239	1.000000	81.0
ycf1_p2	ycf1	2	TTCTCTACGGCGTTTAGGGA	CCCCGTCGTTTGTCTGTAAA	20	20	50.0	50.0	58.2	58.1	21.2	0.0	0.293419	124889	125008	240-240	1.000000	81.0
ycf1_p3	ycf1	3	TCTCTACGGCGTTTAGGGAA	ACAGAATTGACGAAAGGGGG	20	20	50.0	50.0	58.2	57.8	21.2	0.0	0.362261	124889	125008	275-275	1.000000	80.0
ycf1_p4	ycf1	4	TTCTCTACGGCGTTTAGGGA	ACAGAATTGACGAAAGGGGG	20	20	50.0	50.0	58.2	57.8	21.2	0.0	0.362261	124889	125008	276-276	1.000000	80.0
ycf1_p5	ycf1	5	TCTCTACGGCGTTTAGGGAA	GACGAAAGGGGGAATGCTTA	20	20	50.0	50.0	58.2	57.6	21.2	0.0	0.575198	124889	125008	267-267	1.000000	78.4