Hotspot

rpl22

LSC · 79336-79785 · 450 bp

Back to Muhlenbergia

Pi 0.00296

MarkerSeek score 31.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 2
pi: 0.00296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTGCGAACCAAAAGGAATGG	AATTGGGGGAATTTGTGCCT	50.0	45.0	57.9	58.0	744-747 bp	1.0	79.9
rpl22_p2	GTGCGAACCAAAAGGAATGG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	1277-1279 bp	1.0	79.7
rpl22_p3	GTGCGAACCAAAAGGAATGG	TTGGGGGAATTTGTGCCTAC	50.0	50.0	57.9	58.1	742-745 bp	1.0	79.7
rpl22_p4	TAGCGCGAATTGTTTGGAGA	GTTATCCTGCGCTTGGAAGA	45.0	50.0	57.9	58.0	1796-1798 bp	1.0	79.5
rpl22_p5	TAGCGCGAATTGTTTGGAGA	AATTGGGGGAATTTGTGCCT	45.0	45.0	57.9	58.0	1263-1266 bp	1.0	79.5