Hotspot

rpl16

LSC · 77047-78458 · 1412 bp

Back to Muhlenbergia

Pi 0.00142

MarkerSeek score 47.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1412
variable_sites: 3
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGAGAAGGAAAACCTGGGGT	AGAACCTTATTTTCCAAAAATCCCA	50.0	32.0	58.5	57.4	1561-1589 bp	1.0	40.4
rpl16_p2	TTGAGAAGGAAAACCTGGGG	AGAACCTTATTTTCCAAAAATCCCA	50.0	32.0	57.3	57.4	1562-1590 bp	1.0	40.4
rpl16_p3	TGAGAAGGAAAACCTGGGGT	ACCTTATTTTCCAAAAATCCCA	50.0	31.8	58.5	54.1	1558-1586 bp	1.0	40.4
rpl16_p4	TGAGAAGGAAAACCTGGGGT	GAACCTTATTTTCCAAAAATCCCA	50.0	33.3	58.5	56.1	1560-1588 bp	1.0	40.4
rpl16_p5	TGAGAAGGAAAACCTGGGGT	AAGAACCTTATTTTCCAAAAATCCCA	50.0	30.8	58.5	58.0	1562-1590 bp	1.0	40.4