Hotspot

rpl32-trnL-UAG

SSC · 104011-104537 · 527 bp

Back to Muhlenbergia

Pi 0.0164

MarkerSeek score 55.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 527
variable_sites: 13
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCAAAAGGTTTTTCGGGGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	588-601 bp	1.0	79.7
rpl32-trnL-UAG_p2	ACCAAAAGGTTTTTCGGGGT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	581-594 bp	1.0	79.2
rpl32-trnL-UAG_p3	ACCAAAAGGTTTTTCGGGGT	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.1	58.2	602-615 bp	1.0	79.2
rpl32-trnL-UAG_p4	ACCAAAAGGTTTTTCGGGGT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.1	57.8	587-600 bp	1.0	79.1
rpl32-trnL-UAG_p5	ACCAAAAGGTTTTTCGGGGT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	586-599 bp	1.0	79.1