Hotspot

atpB-rbcL

LSC · 53577-54378 · 802 bp

Back to Muhlenbergia

Pi 0.00166

MarkerSeek score 47.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 2
pi: 0.00166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGATCAATACGCCCTGCA	GCTTGAAATCCAACACCTGC	45.0	50.0	57.9	57.9	922-923 bp	1.0	78.7
atpB-rbcL_p2	TTTGATCAATACGCCCTGCA	TTCGTATTCCGGGGTGTAGT	45.0	50.0	57.9	58.1	968-969 bp	1.0	78.1
atpB-rbcL_p3	TATCCAGCACGGGTCCAATA	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	943-944 bp	1.0	78.1
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	GCTTGAAATCCAACACCTGC	50.0	50.0	58.2	57.9	940-941 bp	1.0	77.8
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	GCTTGAAATCCAACACCTGC	55.0	50.0	57.7	57.9	958-959 bp	1.0	77.5