Hotspot

trnT-UGU-trnL-UAA

LSC · 46052-46474 · 423 bp

Back to Muhlenbergia

Pi 0
MarkerSeek score 55.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
423
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 522-524 bp 1.0 80.2
trnT-UGU-trnL-UAA_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 514-516 bp 1.0 79.9
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 492-494 bp 1.0 79.4
trnT-UGU-trnL-UAA_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 512-514 bp 1.0 78.3
trnT-UGU-trnL-UAA_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 521-523 bp 1.0 78.0