Hotspot

psbM-petN

LSC · 16685-17416 · 732 bp

Back to Muhlenbergia

Pi 0.00183

MarkerSeek score 47.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGTCAAAACGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.4	58.1	779-781 bp	1.0	66.6
psbM-petN_p2	AAAACAGCCAGTCAAAACGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.4	58.1	778-780 bp	1.0	66.6
psbM-petN_p3	AAAACAGCCAGTCAAAACGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.4	58.2	837-839 bp	1.0	66.3
psbM-petN_p4	AAAACAGCCAGTCAAAACGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.4	57.8	777-779 bp	1.0	66.2
psbM-petN_p5	AAAACAGCCAGTCAAAACGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.4	58.2	774-776 bp	1.0	65.8