Hotspot

trnS-UGA-psbZ

LSC · 11451-11803 · 353 bp

Back to Muhlenbergia

Pi 0.00378

MarkerSeek score 56.00

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 2
pi: 0.00378
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	473 bp	1.0	78.5
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	475 bp	1.0	78.1
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	493 bp	1.0	77.7
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGGAGAAGC	50.0	55.0	57.9	58.3	495 bp	1.0	77.3
trnS-UGA-psbZ_p5	GCTATCAACCACTCAGCCAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.7	57.8	478 bp	1.0	76.0