Hotspot

ycf1

SSC · 99498-103601 · 4104 bp

Back to Muellerina

Pi 0.0184

MarkerSeek score 50.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4104
variable_sites: 75
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCCAGTCTTTCCATTCCTT	CTTTTGGTCCTCCCCGAAAA	45.0	50.0	56.7	58.0	832-862 bp	1.0	73.3
ycf1_p2	AGCCAGTCTTTCCATTCCTT	TTTTGGTCCTCCCCGAAAAG	45.0	50.0	56.7	58.0	831-861 bp	1.0	73.3
ycf1_p3	AGCCAGTCTTTCCATTCCTT	TCCTCCCCGAAAAGGATCTT	45.0	50.0	56.7	58.0	825-855 bp	1.0	73.2
ycf1_p4	AGCCAGTCTTTCCATTCCTT	TCCCGAGTGGTCTCAAGATT	45.0	50.0	56.7	58.1	1086-1122 bp	1.0	72.2
ycf1_p5	AGCCAGTCTTTCCATTCCTT	TTCCCGAGTGGTCTCAAGAT	45.0	50.0	56.7	58.1	1087-1123 bp	1.0	72.2