Hotspot

ccsA-psaC

SSC · 97850-98163 · 314 bp

Back to Muellerina

Pi 0.0317

MarkerSeek score 67.10

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 314
variable_sites: 9
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TTGGGGATGCTATTTTGGGA	AAGATGTGAATCCGTCTGCC	45.0	50.0	56.8	58.0	475-485 bp	1.0	75.4
ccsA-psaC_p2	GGGGATGCTATTTTGGGATCA	AAGATGTGAATCCGTCTGCC	47.6	50.0	57.8	58.0	473-483 bp	1.0	75.3
ccsA-psaC_p3	TGGGGATGCTATTTTGGGATC	AAGATGTGAATCCGTCTGCC	47.6	50.0	57.8	58.0	474-484 bp	1.0	75.3
ccsA-psaC_p4	TGGGGATGCTATTTTGGGAT	AAGATGTGAATCCGTCTGCC	45.0	50.0	56.5	58.0	474-484 bp	1.0	73.5
ccsA-psaC_p5	TTTGGGGATGCTATTTTGGGA	AAGATGTGAATCCGTCTGCC	42.9	50.0	57.5	58.0	476-486 bp	1.0	73.4