Hotspot

rps12-trnV-GUC

IRb · 85179-86982 · 1804 bp

Back to Muellerina

Pi 0.00833
MarkerSeek score 63.10
Primer available yes
Label rps12-trnV-GUC

Feature Metadata

label_name
rps12-trnV-GUC
feature_type
igs
region
IRb
length_bp
1804
variable_sites
15
pi
0.00833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GUC_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1900-1919 bp 1.0 77.4
rps12-trnV-GUC_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1904-1923 bp 1.0 77.3
rps12-trnV-GUC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1899-1918 bp 1.0 77.3
rps12-trnV-GUC_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1902-1921 bp 1.0 76.4
rps12-trnV-GUC_p5 ACGTAAGGCAGAGTTTGGTT GATGACTTCCACCACGTCAA 45.0 50.0 57.4 57.8 1890-1909 bp 1.0 74.4