Hotspot

ycf15-trnL-UUG

IRb · 81790-82333 · 544 bp

Back to Muellerina

Pi 0.0149

MarkerSeek score 67.60

Primer available yes

Label ycf15-trnL-UUG

Feature Metadata

label_name: ycf15-trnL-UUG
feature_type: igs
region: IRb
length_bp: 544
variable_sites: 8
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-UUG_p1	ACGCATGTCTGATGAAATGGT	AAATGGTAGACACGCGAGAC	42.9	50.0	58.3	58.0	675-683 bp	1.0	71.5
ycf15-trnL-UUG_p2	ACGCATGTCTGATGAAATGGT	TGAAATGGTAGACACGCGAG	42.9	50.0	58.3	58.0	677-685 bp	1.0	71.5
ycf15-trnL-UUG_p3	ACGCATGTCTGATGAAATGGT	GCCTTGGTGGTGAAATGGTA	42.9	50.0	58.3	58.1	687-695 bp	1.0	70.9
ycf15-trnL-UUG_p4	ACGCATGTCTGATGAAATGGT	TGGTGAAATGGTAGACACGC	42.9	50.0	58.3	58.2	680-688 bp	1.0	70.0
ycf15-trnL-UUG_p5	CGCATGTCTGATGAAATGGT	AAATGGTAGACACGCGAGAC	45.0	50.0	56.5	58.0	674-682 bp	1.0	69.8