Hotspot

rpoA-rps11

LSC · 67386-67452 · 67 bp

Back to Muellerina

Pi 0.0317

MarkerSeek score 66.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 2
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CCACTGTAATGTCCGAGTCG	AGAGATGCAGCATTACGAGC	55.0	50.0	58.1	58.1	222-226 bp	1.0	80.3
rpoA-rps11_p2	CCACTGTAATGTCCGAGTCG	GAGATGCAGCATTACGAGCT	55.0	50.0	58.1	58.1	221-225 bp	1.0	80.3
rpoA-rps11_p3	CCACTGTAATGTCCGAGTCG	TGTACGAGATGTAACCCCCA	55.0	50.0	58.1	57.8	172-176 bp	1.0	80.2
rpoA-rps11_p4	CCACTGTAATGTCCGAGTCG	ATGGCTGTCGACCTCCTAAA	55.0	50.0	58.1	58.4	143-147 bp	1.0	79.1
rpoA-rps11_p5	CCACTGTAATGTCCGAGTCG	GCAGAGATGCAGCATTACGA	55.0	50.0	58.1	58.4	224-228 bp	1.0	78.0