Hotspot

psaJ-rpl33

LSC · 56063-56153 · 91 bp

Back to Muellerina

Pi 0.033

MarkerSeek score 63.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 3
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGATTTTTCCCAGATGCGT	CGGGCATCTTTACCACCTTT	45.0	50.0	57.9	58.2	162-163 bp	1.0	79.8
psaJ-rpl33_p2	TCGATTTTTCCCAGATGCGT	GTTACCCGGGCATCTTTACC	45.0	55.0	57.9	58.3	168-169 bp	1.0	78.4
psaJ-rpl33_p3	TGGATCTTTGGCGGGTCTAT	CGGGCATCTTTACCACCTTT	50.0	50.0	58.5	58.2	195-196 bp	1.0	76.4
psaJ-rpl33_p4	TCGATTTTTCCCAGATGCGT	CGTTACCCGGGCATCTTTAC	45.0	55.0	57.9	58.7	169-170 bp	1.0	75.4
psaJ-rpl33_p5	GGATCTTTGGCGGGTCTATT	CGGGCATCTTTACCACCTTT	50.0	50.0	57.4	58.2	194-195 bp	1.0	75.2