Hotspot

atpB-rbcL

LSC · 45664-46259 · 596 bp

Back to Muellerina

Pi 0.0119

MarkerSeek score 66.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 7
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCTACATCCAATACCGGACC	AACACCGGCTTTGAATCCAA	55.0	45.0	57.8	58.3	706-709 bp	1.0	78.0
atpB-rbcL_p2	GCTACATCCAATACCGGACC	TAACACCGGCTTTGAATCCA	55.0	45.0	57.8	57.4	707-710 bp	1.0	75.8
atpB-rbcL_p3	GGGGGAAAAGCTACATCCAA	AACACCGGCTTTGAATCCAA	50.0	45.0	57.5	58.3	715-718 bp	1.0	75.1
atpB-rbcL_p4	GCTACATCCAATACCGGACC	CCGGCTTTGAATCCAACACT	55.0	50.0	57.8	58.8	702-705 bp	1.0	74.4
atpB-rbcL_p5	TATTAGGCATCTTCCCCGGG	AACACCGGCTTTGAATCCAA	55.0	45.0	58.6	58.3	732-735 bp	1.0	74.1