Hotspot

rps4-trnT-ACA

LSC · 41156-41513 · 358 bp

Back to Muellerina

Pi 0.0224

MarkerSeek score 65.90

Primer available yes

Label rps4-trnT-ACA

Feature Metadata

label_name: rps4-trnT-ACA
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 8
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-ACA_p1	AGTTAATCCCGGTAAAGCGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	486-487 bp	1.0	77.7
rps4-trnT-ACA_p2	AGTTAATCCCGGTAAAGCGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	454-455 bp	1.0	76.9
rps4-trnT-ACA_p3	AGTTAATCCCGGTAAAGCGC	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.3	57.5	494-495 bp	1.0	74.7
rps4-trnT-ACA_p4	AGTTAATCCCGGTAAAGCGC	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	484-485 bp	1.0	74.6
rps4-trnT-ACA_p5	AGTTAATCCCGGTAAAGCGC	TCAGAGGTTAGAGCATCGCA	50.0	50.0	58.3	58.5	485-486 bp	1.0	74.6