Hotspot

ycf3-trnS-UCC

LSC · 39612-40304 · 693 bp

Back to Muellerina

Pi 0.0189
MarkerSeek score 66.50
Primer available yes
Label ycf3-trnS-UCC

Feature Metadata

label_name
ycf3-trnS-UCC
feature_type
igs
region
LSC
length_bp
693
variable_sites
12
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-UCC_p1 TGCTTCCCTTTCTCTTGAAGT CCCTCGGTAAACAAAAGCCT 42.9 50.0 57.1 58.1 828-865 bp 1.0 69.5
ycf3-trnS-UCC_p2 TGCTTCCCTTTCTCTTGAAGT TTCCAATGCTACGCCTTGAA 42.9 45.0 57.1 57.8 799-836 bp 1.0 68.7
ycf3-trnS-UCC_p3 TGCTTCCCTTTCTCTTGAAGT GGTGGAAAGAGAGGGATTCG 42.9 55.0 57.1 57.7 850-887 bp 1.0 67.6
ycf3-trnS-UCC_p4 CCCTTTCTCTTGAAGTTGTCG CCCTCGGTAAACAAAAGCCT 47.6 50.0 56.8 58.1 823-860 bp 1.0 67.1
ycf3-trnS-UCC_p5 CCTTTCTCTTGAAGTTGTCGG CCCTCGGTAAACAAAAGCCT 47.6 50.0 56.8 58.1 822-859 bp 1.0 67.1