Hotspot

psbZ-trnG-GGC

LSC · 31504-31770 · 267 bp

Back to Muellerina

Pi 0.0243

MarkerSeek score 70.40

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 6
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCCGATGGTTGGTCAAGTA	CCTTCTCCTTGGCAAAGAGG	50.0	55.0	58.4	58.2	403-415 bp	1.0	80.9
psbZ-trnG-GGC_p2	TTTGCTTCTCCCGATGGTTG	CCTTCTCCTTGGCAAAGAGG	50.0	55.0	58.5	58.2	411-423 bp	1.0	79.9
psbZ-trnG-GGC_p3	CCCGATGGTTGGTCAAGTAA	CCTTCTCCTTGGCAAAGAGG	50.0	55.0	57.5	58.2	402-414 bp	1.0	79.9
psbZ-trnG-GGC_p4	ATTTGCTTCTCCCGATGGTT	CCTTCTCCTTGGCAAAGAGG	45.0	55.0	57.5	58.2	412-424 bp	1.0	79.5
psbZ-trnG-GGC_p5	TTCTCCCGATGGTTGGTCAA	CCTTCTCCTTGGCAAAGAGG	50.0	55.0	58.9	58.2	406-418 bp	1.0	76.2