Hotspot

trnY-UAC-trnE-GAA

LSC · 26441-26497 · 57 bp

Back to Muellerina

Pi 0.0526

MarkerSeek score 73.40

Primer available yes

Label trnY-UAC-trnE-GAA

Feature Metadata

label_name: trnY-UAC-trnE-GAA
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 3
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-UAC-trnE-GAA_p1	ATTTGAACCAGCGTAGGCAT	ATGATGTAGGTATGCCCCCA	45.0	50.0	57.9	57.9	214 bp	1.0	79.0
trnY-UAC-trnE-GAA_p2	ATTTGAACCAGCGTAGGCAT	TGATGTAGGTATGCCCCCAT	45.0	50.0	57.9	57.9	213 bp	1.0	79.0
trnY-UAC-trnE-GAA_p3	ATTTGAACCAGCGTAGGCAT	CTCTCTTTCAAGGAGGCAGC	45.0	55.0	57.9	58.3	173 bp	1.0	78.3
trnY-UAC-trnE-GAA_p4	ATTTGAACCAGCGTAGGCAT	TCGTCTAGTGGTTCAGGACA	45.0	50.0	57.9	57.7	194 bp	1.0	78.0
trnY-UAC-trnE-GAA_p5	CAACGAATTTACAGTCCGCC	TGATGTAGGTATGCCCCCAT	50.0	50.0	57.5	57.9	188 bp	1.0	76.1