Hotspot

trnS-AGC-trnR-AGA

LSC · 6180-6650 · 471 bp

Back to Muellerina

Pi 0.0523

MarkerSeek score 80.80

Primer available yes

Label trnS-AGC-trnR-AGA

Feature Metadata

label_name: trnS-AGC-trnR-AGA
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 23
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnR-AGA_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	532-547 bp	1.0	70.7
trnS-AGC-trnR-AGA_p2	GAAAGAGGGGGATTCGAACC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.0	56.9	578-593 bp	1.0	70.4
trnS-AGC-trnR-AGA_p3	GGAAAGAGGGGGATTCGAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.0	56.9	579-594 bp	1.0	70.4
trnS-AGC-trnR-AGA_p4	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	538-553 bp	1.0	70.0
trnS-AGC-trnR-AGA_p5	GAAAGAGGGGGATTCGAACC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.0	57.9	584-599 bp	1.0	69.7