Hotspot

matK-trnQ-CAA

LSC · 3375-4959 · 1585 bp

Back to Muellerina

Pi 0.0209

MarkerSeek score 56.20

Primer available yes

Label matK-trnQ-CAA

Feature Metadata

label_name: matK-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 1585
variable_sites: 22
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnQ-CAA_p1	TCCCTGTTTACGAGTTCGATC	GGCGTAGTCAAGTGGTAAGG	47.6	55.0	57.5	58.0	1185-1705 bp	1.0	73.2
matK-trnQ-CAA_p2	TCCCTGTTTACGAGTTCGATC	GGGCGTAGTCAAGTGGTAAG	47.6	55.0	57.5	58.0	1186-1706 bp	1.0	73.2
matK-trnQ-CAA_p3	CCCTGTTTACGAGTTCGATCT	GGCGTAGTCAAGTGGTAAGG	47.6	55.0	57.5	58.0	1184-1704 bp	1.0	73.1
matK-trnQ-CAA_p4	CCCTGTTTACGAGTTCGATCT	GGGCGTAGTCAAGTGGTAAG	47.6	55.0	57.5	58.0	1185-1705 bp	1.0	73.1
matK-trnQ-CAA_p5	TCCCTGTTTACGAGTTCGATC	AATTGAAGTGGGGCGTAGTC	47.6	50.0	57.5	57.9	1196-1716 bp	1.0	72.3