Hotspot

ndhA

SSC · 120642-122841 · 2200 bp

Back to Muehlenbeckia

Pi 0.0128

MarkerSeek score 62.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2200
variable_sites: 50
pi: 0.0128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCGCCGGGTTTTATCAATT	TCTTTTTCTTGTTTGGAACAGGT	50.0	34.8	58.9	57.0	2371-2405 bp	1.0	43.3
ndhA_p2	CCGCCGGGTTTTATCAATTT	TCTTTTTCTTGTTTGGAACAGGT	45.0	34.8	57.0	57.0	2370-2404 bp	1.0	42.8
ndhA_p3	GCCGGGTTTTATCAATTTGCA	TCTTTTTCTTGTTTGGAACAGGT	42.9	34.8	58.0	57.0	2368-2402 bp	1.0	42.4
ndhA_p4	CCCGCCGGGTTTTATCAAT	TCTTTTTCTTGTTTGGAACAGGT	52.6	34.8	58.2	57.0	2371-2405 bp	1.0	42.3
ndhA_p5	CCCGCCGGGTTTTATCAATT	TCTTTTTCTTGTTTGGAACAGGTT	50.0	33.3	58.9	57.6	2371-2405 bp	1.0	42.3