Hotspot

rpl32-ndhF

SSC · 129349-130333 · 985 bp

Back to Muehlenbeckia

Pi 0.0163

MarkerSeek score 58.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 985
variable_sites: 28
pi: 0.0163
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CTATTGGCTATTGGGACCGG	GAAAAGCCCACATACGCCTA	55.0	50.0	57.8	58.0	1608-1626 bp	1.0	82.2
rpl32-ndhF_p2	CTATTGGCTATTGGGACCGG	TAGGAAAAGCCCACATACGC	55.0	50.0	57.8	58.0	1611-1629 bp	1.0	82.2
rpl32-ndhF_p3	CTATTGGCTATTGGGACCGG	AAAGCCCACATACGCCTAAG	55.0	50.0	57.8	58.0	1606-1624 bp	1.0	82.1
rpl32-ndhF_p4	CTATTGGCTATTGGGACCGG	AGAAGAAGTCCTGCTCCGAT	55.0	50.0	57.8	58.1	1564-1582 bp	1.0	81.4
rpl32-ndhF_p5	CTATTGGCTATTGGGACCGG	ATCGCGAAATTCGAAACTGC	55.0	45.0	57.8	57.8	2102-2120 bp	1.0	80.4