Hotspot

ndhI-ndhG

SSC · 123458-123804 · 347 bp

Back to Muehlenbeckia

Pi 0.024

MarkerSeek score 70.80

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 347
variable_sites: 15
pi: 0.024
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TGCTTTAGGCCGTTTACCAA	CCCCGAGGCCTCCTAATATA	45.0	55.0	57.7	57.5	517-1896 bp	1.0	75.2
ndhI-ndhG_p2	TTGCTTTAGGCCGTTTACCA	CCCCGAGGCCTCCTAATATA	45.0	55.0	57.7	57.5	518-1897 bp	1.0	75.2
ndhI-ndhG_p3	TGCTTTAGGCCGTTTACCAA	AAATAATACCACCCCGAGGC	45.0	50.0	57.7	57.3	528-1907 bp	1.0	74.3
ndhI-ndhG_p4	TTGCTTTAGGCCGTTTACCA	AAATAATACCACCCCGAGGC	45.0	50.0	57.7	57.3	529-1908 bp	1.0	74.3
ndhI-ndhG_p5	TGCTTTAGGCCGTTTACCAA	CCCCGAGGCCTCCTAATATAA	45.0	52.4	57.7	58.2	517-1896 bp	1.0	70.2