Hotspot

rps4-trnT-UGU

LSC · 49153-49730 · 578 bp

Back to Muehlenbeckia

Pi 0.0228

MarkerSeek score 67.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 25
pi: 0.0228
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCCAGACGTCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	683-703 bp	1.0	82.2
rps4-trnT-UGU_p2	CCCCCAGACGTCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	652-672 bp	1.0	82.1
rps4-trnT-UGU_p3	CCCCCAGACGTCGTATTTTT	TTTATGTGAGCCCGCTTAGC	50.0	50.0	57.9	58.3	702-722 bp	1.0	80.4
rps4-trnT-UGU_p4	TAAAACCCCCAGACGTCGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.4	57.9	688-708 bp	1.0	80.0
rps4-trnT-UGU_p5	TAAAACCCCCAGACGTCGTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.4	58.2	657-677 bp	1.0	80.0