Hotspot

trnT-GGU-psbD

LSC · 34097-35551 · 1455 bp

Back to Muehlenbeckia

Pi 0.0146

MarkerSeek score 64.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1455
variable_sites: 39
pi: 0.0146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1603-1617 bp	1.0	81.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1606-1620 bp	1.0	81.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CGCAACCAGTCATCCATAGT	55.0	50.0	58.1	57.7	1582-1596 bp	1.0	80.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CGCAACCAGTCATCCATAGT	50.0	50.0	58.1	57.7	1585-1599 bp	1.0	80.5
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1602-1616 bp	1.0	80.0