Hotspot

trnY-GUA-trnE-UUC

LSC · 32992-33061 · 70 bp

Back to Muehlenbeckia

Pi 0.031
MarkerSeek score 64.30
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
70
variable_sites
4
pi
0.031
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 TGGATTTGAACCAGCGTAGG CATAGTATGATGGCGGTCGG 50.0 55.0 57.9 58.0 241-246 bp 1.0 82.0
trnY-GUA-trnE-UUC_p2 ATTTGAACCAGCGTAGGCAT CATAGTATGATGGCGGTCGG 45.0 55.0 57.9 58.0 238-243 bp 1.0 81.9
trnY-GUA-trnE-UUC_p3 TGGATTTGAACCAGCGTAGG ATAGTATGATGGCGGTCGGA 50.0 50.0 57.9 58.1 240-245 bp 1.0 81.4
trnY-GUA-trnE-UUC_p4 ATTTGAACCAGCGTAGGCAT ATAGTATGATGGCGGTCGGA 45.0 50.0 57.9 58.1 237-242 bp 1.0 81.3
trnY-GUA-trnE-UUC_p5 AAATGGAATTCTTGGGCCGA CATAGTATGATGGCGGTCGG 45.0 55.0 57.8 58.0 263-268 bp 1.0 80.8