Hotspot

atpF-atpH

LSC · 14304-14739 · 436 bp

Back to Muehlenbeckia

Pi 0.016

MarkerSeek score 68.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 12
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAATCCGAAACTCCCCGC	GCAGAGGGGAAAATACGAGG	50.0	55.0	58.3	58.0	609-620 bp	1.0	80.9
atpF-atpH_p2	CCCGGGGAAACGAAAGAATC	GCAGAGGGGAAAATACGAGG	55.0	55.0	58.9	58.0	576-587 bp	1.0	75.9
atpF-atpH_p3	TTAAATCCGAAACTCCCCGC	CCGAAGCAGAGGGGAAAATA	50.0	50.0	58.3	57.3	614-625 bp	1.0	75.6
atpF-atpH_p4	TTAAATCCGAAACTCCCCGC	AGCAGAGGGGAAAATACGAG	50.0	50.0	58.3	57.0	610-621 bp	1.0	73.4
atpF-atpH_p5	CCCGGGGAAACGAAAGAAT	GCAGAGGGGAAAATACGAGG	52.6	55.0	57.7	58.0	576-587 bp	1.0	73.1