Hotspot

trnK-UUU-rps16

LSC · 4148-5003 · 856 bp

Back to Muehlenbeckia

Pi 0.0183
MarkerSeek score 64.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
856
variable_sites
29
pi
0.0183
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1026-1043 bp 1.0 80.3
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1027-1044 bp 1.0 80.3
trnK-UUU-rps16_p3 GGACGAATCCCTTGCTTCAT CGCTCAACCTACTGGAACTG 50.0 55.0 58.0 58.3 1029-1046 bp 1.0 80.3
trnK-UUU-rps16_p4 AACTCTACCGATGGTGTGGA CGCTCAACCTACTGGAACTG 50.0 55.0 58.1 58.3 1046-1063 bp 1.0 80.1
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA TTCGCCCTAATGAAACGACA 50.0 45.0 58.0 57.5 967-984 bp 1.0 79.1