Hotspot

trnH-GUG-psbA

LSC · 80-289 · 210 bp

Back to Muehlenbeckia

Pi 0.0397

MarkerSeek score 79.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 14
pi: 0.0397
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	325-349 bp	1.0	82.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	339-363 bp	1.0	82.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	338-362 bp	1.0	82.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	340-364 bp	1.0	82.1
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.5	58.0	354-378 bp	1.0	79.2