Hotspot

ycf1

SSC · 122406-128072 · 5667 bp

Pi 0.0031

MarkerSeek score 39.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5667
variable_sites: 39
pi: 0.0031
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGGCCTCAATATCGACT	TCAAGGAAATAAGAATCAAGGA	50.0	31.8	57.9	52.8	203 bp	1.0	41.6
ycf1_p2	TCCAGGCCTCAATATCGACT	TCTTTCAAGGAAATAAGAATCAAGGA	50.0	30.8	57.9	56.8	207 bp	1.0	41.5
ycf1_p3	TCCAGGCCTCAATATCGACT	CTCTTTCAAGGAAATAAGAATCAAGGA	50.0	33.3	57.9	57.8	208 bp	1.0	41.5
ycf1_p4	TCCAGGCCTCAATATCGACT	TCTCTTTCAAGGAAATAAGAATCAAGG	50.0	33.3	57.9	57.8	209 bp	1.0	41.5
ycf1_p5	GTCCAGGCCTCAATATCGAC	TCTTTCAAGGAAATAAGAATCAAGGA	55.0	30.8	57.8	56.8	208 bp	1.0	41.5