Hotspot

rps16

LSC · 4825-5959 · 1135 bp

Pi 0.00114

MarkerSeek score 44.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1135
variable_sites: 3
pi: 0.00114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATATACAAGCCGCTACCCCC	GCAGCTGAACCGATGACTAT	55.0	50.0	59.0	57.8	1242-1244 bp	1.0	70.3
rps16_p2	TATACAAGCCGCTACCCCC	GCAGCTGAACCGATGACTAT	57.9	50.0	58.6	57.8	1241-1243 bp	1.0	66.0
rps16_p3	TATATACAAGCCGCTACCCC	GCAGCTGAACCGATGACTAT	50.0	50.0	56.3	57.8	1243-1245 bp	1.0	64.6
rps16_p4	ATATACAAGCCGCTACCCCC	AAGTGTAGATTGCGACGTCT	55.0	45.0	59.0	57.0	1266-1268 bp	1.0	64.0
rps16_p5	TATATACAAGCCGCTACCCCC	GCAGCTGAACCGATGACTAT	52.4	50.0	58.8	57.8	1243-1245 bp	1.0	63.9