Hotspot

rpoC2

LSC · 16509-20705 · 4197 bp

Pi 0.00114

MarkerSeek score 45.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4197
variable_sites: 13
pi: 0.00114
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCATTCGAAAACAATTGGATGA	GGCAAACGGTTTGATTCGAC	39.1	50.0	57.9	58.3	282 bp	1.0	54.0
rpoC2_p2	CCCATTCGAAAACAATTGGATGA	GCAAACGGTTTGATTCGACA	39.1	45.0	57.9	57.6	281 bp	1.0	53.4
rpoC2_p3	ATCAACGATTGGAATCCCTT	GGCAAACGGTTTGATTCGAC	40.0	50.0	54.7	58.3	249 bp	1.0	52.2
rpoC2_p4	ATCAACGATTGGAATCCCTT	GCAAACGGTTTGATTCGACA	40.0	45.0	54.7	57.6	248 bp	1.0	51.6
rpoC2_p5	CCCATTCGAAAACAATTGGATGA	TCTAGTCAAGAAGCTCGTGC	39.1	50.0	57.9	57.4	320 bp	1.0	51.3