Hotspot

matK

LSC · 2017-3561 · 1545 bp

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Pi 0.0014
MarkerSeek score 44.70
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1545
variable_sites
5
pi
0.0014
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 AGTGGAGATCCCATTTTGATCTT AACCCCCGAATCTTCTACCT 39.1 50.0 57.5 57.7 1663 bp 1.0 50.4
matK_p2 AGTGGAGATCCCATTTTGATCTT ACCCCCGAATCTTCTACCTT 39.1 50.0 57.5 57.7 1662 bp 1.0 50.4
matK_p3 GTGGAGATCCCATTTTGATCTT AACCCCCGAATCTTCTACCT 40.9 50.0 56.1 57.7 1662 bp 1.0 47.0
matK_p4 GTGGAGATCCCATTTTGATCTT ACCCCCGAATCTTCTACCTT 40.9 50.0 56.1 57.7 1661 bp 1.0 47.0
matK_p5 AGTGGAGATCCCATTTTGATCTTT ACCCCCGAATCTTCTACCTT 37.5 50.0 58.2 57.7 1662 bp 1.0 44.8