Hotspot

accD-psaI

LSC · 58760-59481 · 722 bp

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Pi 0.00243
MarkerSeek score 46.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
722
variable_sites
4
pi
0.00243
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TAAGCTCCACGCCTTCTTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 826-838 bp 1.0 80.7
accD-psaI_p2 CTCCACGCCTTCTTTCCTTT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 822-834 bp 1.0 80.6
accD-psaI_p3 CGACTTAATCGTACCGCGTA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.6 58.0 877-889 bp 1.0 78.4
accD-psaI_p4 TAAGCTCCACGCCTTCTTTC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.9 57.9 832-844 bp 0.9 75.9
accD-psaI_p5 CTCCACGCCTTCTTTCCTTT AAGAAGCCATTGCAATTGCC 50.0 45.0 58.1 57.9 828-840 bp 0.9 75.9