Hotspot

rps4-trnT-UGU

LSC · 45778-46248 · 471 bp

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Pi 0.00635
MarkerSeek score 49.90
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
471
variable_sites
6
pi
0.00635
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TCTAAGATCACTCCCGGCTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 631-635 bp 1.0 78.8
rps4-trnT-UGU_p2 TTCTAAGATCACTCCCGGCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.8 57.9 632-636 bp 1.0 78.8
rps4-trnT-UGU_p3 TCTAAGATCACTCCCGGCTT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 599-603 bp 1.0 78.0
rps4-trnT-UGU_p4 TTCTAAGATCACTCCCGGCT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.8 57.8 600-604 bp 1.0 78.0
rps4-trnT-UGU_p5 GTTAGTCCTGGTAAAGCCCC CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.6 57.9 597-601 bp 1.0 76.8