Hotspot

ycf3-trnS-GGA

LSC · 44030-44865 · 836 bp

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Pi 0.00136
MarkerSeek score 47.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
836
variable_sites
3
pi
0.00136
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG AAGGTTCGGAAAGAGAGGGA 50.0 50.0 57.1 58.0 1032-1035 bp 1.0 73.3
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT AAGGTTCGGAAAGAGAGGGA 50.0 50.0 57.1 58.0 1031-1034 bp 1.0 73.3
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1006-1009 bp 1.0 72.8
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1005-1008 bp 1.0 72.8
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TCGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 57.9 1027-1030 bp 1.0 72.4