Hotspot

trnK-UUU-rps16

LSC · 4293-4824 · 532 bp

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Pi 0.00526
MarkerSeek score 39.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
532
variable_sites
6
pi
0.00526
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGCACTTAAAAGCCGAGTAC TCCTTGAAAAAGGGGCTCAA 50.0 45.0 57.2 57.2 671-675 bp 1.0 69.2
trnK-UUU-rps16_p2 CGCACTTAAAAGCCGAGTAC TTCCTTGAAAAAGGGGCTCA 50.0 45.0 57.2 57.2 672-676 bp 1.0 69.2
trnK-UUU-rps16_p3 CGCACTTAAAAGCCGAGTAC CCTTGAAAAAGGGGCTCAAC 50.0 50.0 57.2 57.2 670-674 bp 1.0 68.7
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC CTTGAAAAAGGGGCTCAACC 50.0 50.0 57.2 57.2 669-673 bp 1.0 68.7
trnK-UUU-rps16_p5 AGCCGAGTACTCTACCATTGA TCCTTGAAAAAGGGGCTCAA 47.6 45.0 58.0 57.2 661-665 bp 1.0 67.3