Hotspot

trnH-GUG-psbA

LSC · 76-417 · 342 bp

Back to Mosla

Pi 0.0121
MarkerSeek score 33.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
342
variable_sites
11
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGACCTAGCTGCTATCGAAG 55.0 50.0 58.1 56.2 400-401 bp 1.0 67.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGACCTAGCTGCTATCGAAG 50.0 50.0 57.9 56.2 414-415 bp 1.0 67.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGACCTAGCTGCTATCGAAG 50.0 50.0 57.9 56.2 413-414 bp 1.0 67.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGACCTAGCTGCTATCGAAG 50.0 50.0 57.9 56.2 415-416 bp 1.0 67.0
trnH-GUG-psbA_p5 GCCTTGATCCACTTGGCTAC AGACCTAGCTGCTATCGAAG 55.0 50.0 58.6 56.2 405-406 bp 1.0 63.2