Hotspot

ccsA-ndhD

SSC · 120285-120582 · 298 bp

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Pi 0.00671
MarkerSeek score 48.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
298
variable_sites
10
pi
0.00671
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCCGCAATTGTAGCCTCG TCAGTTGACAAAGTCGAAGCT 50.0 42.9 58.7 57.8 448-462 bp 1.0 69.2
ccsA-ndhD_p2 ATTCCGCAATTGTAGCCTCG AGTTGACAAAGTCGAAGCTCT 50.0 42.9 58.7 57.5 446-460 bp 1.0 66.9
ccsA-ndhD_p3 CCGCAATTGTAGCCTCGAT TCAGTTGACAAAGTCGAAGCT 52.6 42.9 58.0 57.8 445-459 bp 1.0 66.7
ccsA-ndhD_p4 ATTCCGCAATTGTAGCCTCG CAGTTGACAAAGTCGAAGCTC 50.0 47.6 58.7 57.5 447-461 bp 1.0 66.5
ccsA-ndhD_p5 GCAATTGTAGCCTCGATGGG TCAGTTGACAAAGTCGAAGCT 55.0 42.9 59.1 57.8 443-457 bp 1.0 66.4