Hotspot

rpl32-trnL-UAG

SSC · 117460-119131 · 1672 bp

Back to Morus

Pi 0.00178
MarkerSeek score 59.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1672
variable_sites
9
pi
0.00178
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1701-1792 bp 1.0 83.0
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1702-1793 bp 1.0 82.9
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TTTAGGAGATGTCATGCCGC 45.0 50.0 57.8 58.0 1766-1857 bp 1.0 82.6
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1711-1802 bp 1.0 82.0
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 1709-1800 bp 1.0 81.4