Hotspot

psbE-petL

LSC · 68256-69469 · 1214 bp

Back to Morus

Pi 0.0138
MarkerSeek score 67.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1214
variable_sites
77
pi
0.0138
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA GTCGTATCTTGTTCAGACCGA 45.0 47.6 58.0 57.5 1339-1438 bp 1.0 76.5
psbE-petL_p2 TGACAAATAACCAACCCGCA AGTCGTATCTTGTTCAGACCG 45.0 47.6 58.0 57.5 1340-1439 bp 1.0 76.4
psbE-petL_p3 CAACCCGCAATGAATAGGGA GTCGTATCTTGTTCAGACCGA 50.0 47.6 57.9 57.5 1328-1427 bp 1.0 76.2
psbE-petL_p4 CAACCCGCAATGAATAGGGA AGTCGTATCTTGTTCAGACCG 50.0 47.6 57.9 57.5 1329-1428 bp 1.0 76.2
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGTTCAGACCGA 50.0 47.6 57.8 57.5 1251-1350 bp 1.0 75.7