Pi
0.0564
MarkerSeek score
91.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CGATGACCCTCGCATTACAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1150-1191 bp | 1.0 | 91.4 |
| trnT-UGU-trnL-UAA_p2 | CGATGACCCTCGCATTACAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1158-1199 bp | 1.0 | 90.9 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1128-1169 bp | 1.0 | 90.9 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1136-1177 bp | 1.0 | 90.4 |
| trnT-UGU-trnL-UAA_p5 | CGATGACCCTCGCATTACAA | AGTCCGTAGCGTCTACCAAT | 50.0 | 50.0 | 58.1 | 58.2 | 1164-1205 bp | 1.0 | 89.6 |