Hotspot

rps4-trnT-UGU

LSC · 48864-49350 · 487 bp

Back to Morus

Pi 0.0353
MarkerSeek score 76.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
487
variable_sites
85
pi
0.0353
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CGGGGCGTAATTGGTTTCTA TTGTAATGCGAGGGTCATCG 50.0 50.0 58.0 58.1 497-654 bp 1.0 91.3
rps4-trnT-UGU_p2 CGGGGCGTAATTGGTTTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 519-676 bp 1.0 90.7
rps4-trnT-UGU_p3 CCCCCAGACGACGTATTTTT TTGTAATGCGAGGGTCATCG 50.0 50.0 57.9 58.1 431-588 bp 1.0 90.6
rps4-trnT-UGU_p4 CCCCCAGACGACGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 453-610 bp 1.0 90.1
rps4-trnT-UGU_p5 GAAACGAGGCCCTCTGTAAC TTGTAATGCGAGGGTCATCG 55.0 50.0 58.3 58.1 534-566 bp 0.9 76.6