Hotspot

trnG-GCC-trnfM-CAU

LSC · 38951-39177 · 227 bp

Back to Morus

Pi 0.016
MarkerSeek score 59.00
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
227
variable_sites
18
pi
0.016
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 245-314 bp 1.0 88.3
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 246-315 bp 1.0 88.3
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 243-312 bp 1.0 87.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 244-313 bp 1.0 87.2
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC AGTTCTTCTCTTCAACGCGG 50.0 50.0 58.1 58.2 300-369 bp 0.9 77.8