Hotspot

atpH-atpI

LSC · 14688-15894 · 1207 bp

Back to Morus

Pi 0.000552
MarkerSeek score 47.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1207
variable_sites
2
pi
0.000552
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.0 57.9 1281-1294 bp 1.0 80.0
atpH-atpI_p2 TACCTTGACCAACTCCAGGT TTTTGCAACTTTAGCTGCGG 50.0 45.0 57.9 57.9 1337-1350 bp 1.0 79.3
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.2 57.9 1282-1295 bp 1.0 78.8
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT ACAAGTGGCATTCAAGCTCT 45.0 45.0 58.0 57.7 1304-1317 bp 1.0 78.8
atpH-atpI_p5 GCAGTACCTTGACCAACTCC TTTTGCAACTTTAGCTGCGG 55.0 45.0 58.2 57.9 1341-1354 bp 1.0 78.7