Hotspot

trnH-GUG-psbA

LSC · 99-473 · 375 bp

Back to Morus

Pi 0.00107
MarkerSeek score 47.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
375
variable_sites
2
pi
0.00107
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GGAAGTTATGCACGAGCGTA 55.0 50.0 58.1 58.1 335-477 bp 1.0 82.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GGAAGTTATGCACGAGCGTA 50.0 50.0 57.9 58.1 349-491 bp 1.0 82.6
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GGAAGTTATGCACGAGCGTA 50.0 50.0 57.9 58.1 348-490 bp 1.0 82.6
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GGAAGTTATGCACGAGCGTA 50.0 50.0 57.9 58.1 350-492 bp 1.0 82.6
trnH-GUG-psbA_p5 TTAATGGGTATGGGCGAACG GGAAGTTATGCACGAGCGTA 50.0 50.0 58.0 58.1 400-541 bp 0.9 79.1