primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GGAAGTTATGCACGAGCGTA	20	20	55.0	50.0	58.1	58.1	0.0	2.4	0.211647	99	473	335-477	1.000000	82.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.216009	99	473	349-491	1.000000	82.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.217516	99	473	348-490	1.000000	82.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	57.9	58.1	0.0	2.4	0.217516	99	473	350-492	1.000000	82.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TTAATGGGTATGGGCGAACG	GGAAGTTATGCACGAGCGTA	20	20	50.0	50.0	58.0	58.1	0.0	2.4	0.126431	99	473	400-541	0.900000	79.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ACGTTGCCTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.170863	6650	7631	1028-1048	1.000000	80.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GTTGCCTTCTACCACATCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.170863	6650	7631	1026-1046	1.000000	80.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CACGTTGCCTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.267690	6650	7631	1029-1049	1.000000	79.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6650	7631	1050-1070	1.000000	78.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6650	7631	1049-1069	1.000000	78.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14688	15894	1281-1294	1.000000	80.0
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.245019	14688	15894	1337-1350	1.000000	79.3
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	14688	15894	1282-1295	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	ACAAGTGGCATTCAAGCTCT	20	20	45.0	45.0	58.0	57.7	1.4	0.0	0.321532	14688	15894	1304-1317	1.000000	78.8
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.323056	14688	15894	1341-1354	1.000000	78.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	38064	38879	594-984	1.000000	85.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	38064	38879	595-985	1.000000	85.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GCTTCTCCTGATGGTTGGTT	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.265251	38064	38879	592-982	1.000000	85.9
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTT	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.265251	38064	38879	593-983	1.000000	85.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCCTGATGGTTGGTTGGGTA	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.309943	38064	38879	587-977	1.000000	85.5
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	38951	39177	245-314	1.000000	88.3
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	38951	39177	246-315	1.000000	88.3
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.237587	38951	39177	243-312	1.000000	87.2
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GAGAAGATGCGGGTTCGATT	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.237587	38951	39177	244-313	1.000000	87.2
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	AGAAGATGCGGGTTCGATTC	AGTTCTTCTCTTCAACGCGG	20	20	50.0	50.0	58.1	58.2	0.0	12.5	0.274150	38951	39177	300-369	0.900000	77.8
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CGGGGCGTAATTGGTTTCTA	TTGTAATGCGAGGGTCATCG	20	20	50.0	50.0	58.0	58.1	0.0	0.4	0.092415	48864	49350	497-654	1.000000	91.3
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.159743	48864	49350	519-676	1.000000	90.7
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CCCCCAGACGACGTATTTTT	TTGTAATGCGAGGGTCATCG	20	20	50.0	50.0	57.9	58.1	0.0	0.4	0.168948	48864	49350	431-588	1.000000	90.6
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.236277	48864	49350	453-610	1.000000	90.1
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GAAACGAGGCCCTCTGTAAC	TTGTAATGCGAGGGTCATCG	20	20	55.0	50.0	58.3	58.1	9.5	0.4	0.342192	48864	49350	534-566	0.900000	76.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CGATGACCCTCGCATTACAA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070035	49424	50542	1150-1191	1.000000	91.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.134844	49424	50542	1158-1199	1.000000	90.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49424	50542	1128-1169	1.000000	90.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49424	50542	1136-1177	1.000000	90.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGATGACCCTCGCATTACAA	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	58.1	58.2	0.0	10.2	0.305220	49424	50542	1164-1205	1.000000	89.6
trnL-UAA_p1	trnL-UAA	1	ACAGTTTTCAATATGAGAACCGC	ACGAATTTTGAACCGCTAACG	23	21	39.1	42.9	57.7	57.8	9.4	0.0	4.474418	50543	51126	754-756	0.900000	40.5
trnL-UAA_p2	trnL-UAA	2	ACAGTTTTCAATATGAGAACCGC	TGAACCGCTAACGAAATGAGA	23	21	39.1	42.9	57.7	57.7	9.4	0.0	4.603730	50543	51126	746-748	0.900000	39.5
trnL-UAA_p3	trnL-UAA	3	ACAGTTTTCAATATGAGAACCGC	CCGCTAACGAAATGAGAAGG	23	20	39.1	50.0	57.7	56.6	9.4	0.0	4.672619	50543	51126	742-744	0.900000	38.9
trnL-UAA_p4	trnL-UAA	4	ACAGTTTTCAATATGAGAACCGC	ACCGCTAACGAAATGAGAAGG	23	21	39.1	47.6	57.7	58.4	9.4	0.0	4.678760	50543	51126	743-745	0.900000	38.9
trnL-UAA_p5	trnL-UAA	5	ACAGTTTTCAATATGAGAACCGC	CCGCTAACGAAATGAGAAGGT	23	21	39.1	47.6	57.7	58.4	9.4	0.0	4.678760	50543	51126	742-744	0.900000	38.9
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	51127	51500	423-432	1.000000	88.2
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	CAAATTGCTCATGTGCCAGG	20	20	50.0	50.0	57.5	58.0	0.0	16.6	0.478992	51127	51500	486-495	1.000000	88.2
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	51127	51500	459-468	1.000000	87.8
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	AAATTGCTCATGTGCCAGGA	20	20	50.0	45.0	57.5	58.1	0.0	16.6	0.546922	51127	51500	485-494	1.000000	87.6
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	AATTGCTCATGTGCCAGGAA	20	20	50.0	45.0	57.5	58.1	0.0	16.6	0.546922	51127	51500	484-493	1.000000	87.6
ycf4-cemA_p1	ycf4-cemA	1	CAGAAAGCTGCTGAATTGGC	AGAGAGATCCACCAAGGCAA	20	20	50.0	50.0	58.0	58.3	1.4	0.0	0.346367	63860	64813	1081-1084	1.000000	77.6
ycf4-cemA_p2	ycf4-cemA	2	CATTCCCTTGACTCGTACCG	AGAGAGATCCACCAAGGCAA	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.415966	63860	64813	1130-1133	1.000000	77.0
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	AGAGAGATCCACCAAGGCAA	20	20	45.0	50.0	58.1	58.3	3.3	0.0	0.434250	63860	64813	1066-1069	1.000000	76.9
ycf4-cemA_p4	ycf4-cemA	4	ATTCCCTTGACTCGTACCGA	AGAGAGATCCACCAAGGCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.495169	63860	64813	1129-1132	1.000000	76.4
ycf4-cemA_p5	ycf4-cemA	5	TTCCCTTGACTCGTACCGAT	AGAGAGATCCACCAAGGCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.495169	63860	64813	1128-1131	1.000000	76.4
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GTCGTATCTTGTTCAGACCGA	20	21	45.0	47.6	58.0	57.5	0.0	0.0	1.500062	68256	69469	1339-1438	1.000000	76.5
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AGTCGTATCTTGTTCAGACCG	20	21	45.0	47.6	58.0	57.5	0.0	0.0	1.506520	68256	69469	1340-1439	1.000000	76.4
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGTTCAGACCGA	20	21	50.0	47.6	57.9	57.5	6.4	0.0	1.534056	68256	69469	1328-1427	1.000000	76.2
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGTTCAGACCG	20	21	50.0	47.6	57.9	57.5	6.4	0.0	1.540515	68256	69469	1329-1428	1.000000	76.2
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAGACCGA	20	21	50.0	47.6	57.8	57.5	24.9	0.0	1.639065	68256	69469	1251-1350	1.000000	75.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.182194	117460	119131	1701-1792	1.000000	83.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.183564	117460	119131	1702-1793	1.000000	82.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCATTGAAAGCTTTT	TTTAGGAGATGTCATGCCGC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.220804	117460	119131	1766-1857	1.000000	82.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	117460	119131	1711-1802	1.000000	82.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.8	57.8	14.0	0.0	0.370658	117460	119131	1709-1800	1.000000	81.4
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCCGCAATTGTAGCCTCG	TCAGTTGACAAAGTCGAAGCT	20	21	50.0	42.9	58.7	57.8	7.4	0.0	1.879902	120285	120582	448-462	1.000000	69.2
ccsA-ndhD_p2	ccsA-ndhD	2	ATTCCGCAATTGTAGCCTCG	AGTTGACAAAGTCGAAGCTCT	20	21	50.0	42.9	58.7	57.5	7.4	0.0	2.161316	120285	120582	446-460	1.000000	66.9
ccsA-ndhD_p3	ccsA-ndhD	3	CCGCAATTGTAGCCTCGAT	TCAGTTGACAAAGTCGAAGCT	19	21	52.6	42.9	58.0	57.8	7.4	0.0	2.187354	120285	120582	445-459	1.000000	66.7
ccsA-ndhD_p4	ccsA-ndhD	4	ATTCCGCAATTGTAGCCTCG	CAGTTGACAAAGTCGAAGCTC	20	21	50.0	47.6	58.7	57.5	7.4	0.0	2.212315	120285	120582	447-461	1.000000	66.5
ccsA-ndhD_p5	ccsA-ndhD	5	GCAATTGTAGCCTCGATGGG	TCAGTTGACAAAGTCGAAGCT	20	21	55.0	42.9	59.1	57.8	7.4	0.0	2.232199	120285	120582	443-457	1.000000	66.4