Hotspot

matK

LSC · 2037-3557 · 1521 bp

Back to Moringa

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1521
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 ACTACACGGTCCCATAACCA ACCCAATAAACACCCGTTCA 50.0 45.0 58.1 57.3 1581 bp 1.0 74.2
matK_p2 CACTACACGGTCCCATAACC ACCCAATAAACACCCGTTCA 55.0 45.0 57.7 57.3 1582 bp 1.0 72.3
matK_p3 ACTACACGGTCCCATAACCA AACCCAATAAACACCCGTTCA 50.0 42.9 58.1 58.1 1582 bp 1.0 71.1
matK_p4 ACTACACGGTCCCATAACCA ACCCAATAAACACCCGTTCAA 50.0 42.9 58.1 58.1 1581 bp 1.0 71.1
matK_p5 CACTACACGGTCCCATAACC ACCCAATAAACACCCGTTCAA 55.0 42.9 57.7 58.1 1582 bp 1.0 69.1